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Pune University - SY BSc (Sem - I) MICROBIOLOGY - II April 2010

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Total No. of Questions : 4] P275 [Total No. of Pages : 2 [3717] - 118 S.Y. B.Sc. MICROBIOLOGY MB - 212 : Microbial Genetics (Paper - II) (Sem. - I) (New Course) Time : 2 Hours] Instructions to the candidates : 1) All questions are compulsory. 2) Figures to the right indicate full marks. 3) Draw neat labelled diagrams wherever necessary. [Max. Marks : 40 Q1) Attempt the following (All subquestions are compulsory) : a) Draw the structure of thymine. b) What is the conservative mode of DNA replication? c) Write two examples of alkylating agents. d) What are transversion mutations? e) Define transcription. f) What is initiation codon? g) Define Okazaki fragments . h) Intercalating agents cause __________ i) Frameshift mutations. ii) Nonsense mutations. iii) Mis-sense mutations. i) Write the function of DNA gyrases in supercoiling of DNA. j) ________ causes formation of pyrimidine dimers i) Ionising radiations. ii) Non ionising radiations. iii) Base analogues. [10] Q2) Attempt any two of the following : [10] a) Write the properties of genetic code. b) Explain J Cairn s experiment to prove DNA as genetic material. c) Schematically represent semiconservative, conservative and dispersive mode of DNA replication. P.T.O.

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Additional Info : S.Y. B.Sc. Microbiology (Semister - I), MB - 212 : Microbial Genetics (Paper - II) ((New Course), Pune University
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