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2003 Course Enzyme & Fermentation Engineering

3 pages, 35 questions, 1 questions with responses, 1 total responses,    0    0
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Total No. of Questions :11] [Total No. of Pages : 3 [3864] - 432 B.E. (Biotechnology) ENZYME AND FERMENTATION ENGINEERING (2003 Course) (Sem.- I) (416282) P1111 Time : 3 Hours] [Max. Marks : 100 Instructions to the candidates: 1) Answer three questions from Section I and three questions from Section II. 2) Answers to the two sections should be written in separate answer books. 3) Neat diagrams should be drawn whenever necessary. 4) Figures to the right indicate full marks. SECTION - I Q1) a) Explain the mechanism of different types of reversible enzyme inhibition and how it affects the kinetics of reaction in terms of the Michaelis Menten constants? [10] b) What is dilution rate? Discuss the effect of dilution rate on biomass concentration in a chemostat. [6] OR Q2) a) Prove that the dilution rate equals the specific growth rate for balanced growth conditions in a chemostat. [5] b) Explain the significance of constants in the Michaelis Menten equation for characterizing enzyme kinetics. [5] c) An inhibitor (I) is added to the enzymatic reaction at a level of 1 gram/lit. The following data was obtained for Km = 9.2 gram S/lit. Is the inhibitor competitive or non-competitive? [6] V 0.909 0.658 0.493 0.4 0.33 0.289 0.227 g/l-min S g/l 20 10 6.67 5 4 3.33 2.5 Q3) a) What is submerged liquid fermentation? Explain with characteristics and applications. [6] b) Describe in detail the continuous mode of fermenter operation. [6] c) Explain the construction, working and applications of hollow fibre bioreactor. [6] P.T.O.

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