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ISC Class XII Board Specimen 2012 : Biotechnology Paper 2

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ISC 12th
Indian School Certificate Examination (ISC), New Delhi
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ISC SPECIMEN QUESTION PAPER FOR 2012 EXAMINATION BIOTECHNOLOGY Paper 2 (Practical) (Three hours) (Candidates are allowed additional 15 minutes for only reading the paper. They must NOT start writing during this time) ______________________________________________________________________________ This paper comprises two sections Section A and Section B. Attempt Question 1 (compulsory) and any two other questions from Section A. Section B is compulsory. The intended marks for questions or parts of questions are given in brackets [ ]. ______________________________________________________________________________ SECTION A Question 1 (Compulsory) (a) You are provided with two stock solutions 1% coconut milk; 1% Sucrose solution and 1 gm of agarose powder. Prepare 100 ml of tissue culture media in a 250 ml flask using the given composition: [10 ml coconut milk + 10 ml sucrose solution + 1% agarose powder and raise the volume to 100 ml using distilled water]. Adjust the media at a pH 6.8 using a pH meter or a pH paper. Sterilize the media using an autoclave. Pour about 4ml of the media in two test tubes to prepare a slant. Answer the questions that follow: (i) What is the role of sucrose and agarose in the given media? (ii) Name the kind of media prepared above. (iii) Explain the principle on which an autoclave works. (iv) What is the purpose of preparing a slant? [1] [1] [1] [1] (b) Perform the experiment described below and answer the questions that follow: Take a ripe mashed banana (about 2 piece) into a beaker and add 30 ml of extraction fluid (E.F) provided to you. Incubate the beaker at 60 degrees C for 15 minutes. Stir gently with a glass rod. Filter 5 ml of the cooled content into a test tube and add 5 ml of cold 90 % alcohol to it. Allow the test tube to stand for 10 minutes. (i) What do you observe in the test tube after 10 minutes? (ii) Give two uses of genomic DNA in molecular biology experiments. (iii) Name a major contaminant found in the above sample. (iv) Name two equipments used for quantitative estimation of DNA. [1] [1] [1] [1] ISC Specimen Question Paper - 2012

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