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2003 Course Enzyme & Fermentation Eng.

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Total No. of Questions : 11] [Total No. of Pages : 3 P1385 [3764] - 452 B.E. (Biotechnology) ENZYME AND FERMENTATION ENGINEERING (2003 Course) (416282) (Sem. - I) Time : 3 Hours] [Max. Marks : 100 Instructions to the candidates: 1) Answer three questions from Section - I and three questions from Section - II. 2) Answers to the two sections should be written in separate answer books. 3) Neat diagrams should be drawn wherever necessary. 4) Figures to the right indicate full marks. SECTION - I Q1) a) b) c) Derive the Michaelis-Menten equation for kinetics of enzyme acting on one substrate. State the assumptions made and explain the significance of the constants in the equation. [8] Explain the various mechanisms leading to enzyme deactivation. [5] Write a note on factors affecting microbial growth kinetics. [5] OR Q2) a) b) c) Q3) a) b) c) Explain the mechanism of substrate activation and inhibition. Also give the expressions representing the respective kinetics. [6] Describe the kinetics of enzyme acting on two substrates. [8] Explain the effect of temperature on enzyme kinetics. Also give the related expressions. [4] What is solid state fermentation? Enlist the characteristics, applications, advantages and disadvantages of solid state fermentation. [8] Describe in detail the continuous mode of operation of a fermenter system. [4] Write a note on the construction, working and applications of an airlift bioreactor. [4] P.T.O.

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